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Employment of hyphenated approach for metabolomic fingerprinting of phenolics from Torilis leptophylla roots
1 , 2 , * 1 , 3
1  Department of Chemistry, Quaid-i-Azam University, Islamabad-45320, PAKISTAN
2  University of Tunis El Manar, Institut Pasteur de Tunis, Laboratory of BioInformatics, BioMathematics & BioStatistics, Tunisia
3  Sharif Medical & Dental College, Jati Umra, Sharif Medical City Road, Lahore, Pakistan


Torilis leptophylla synonymously called bristle fruit hedge parsley belongs to the genus Torilis. It is widely appraised for its folkloric use in Indo-Pak subcontinent for its potential to cope with liver and gastrointestinal diseases. In order to discern the full medicinal prospects of Torilis leptophylla roots, it is required to have a complete picture of its phytoconstituents therefore comprehensive HPLC-MS profiling is hereby reported for the first time that led to the identification of 11 compounds in the roots of Torilis leptophylla including a C-linked glycoside named          as Flavone-6-C-β-D-xylopyranosyl-(1®4)-α-L-rhamnopyranosyl-(1®4)-α-L-rhamnopyranosyl-(1®4)-β-D-glucopyranoside. This compound was identified for the first time in this genus through combined application of TLC, PC and HPLC-DAD-ESI-MS analyses. So the present study paves way for establishing the identity of secondary metabolites, metabolomic fingerprinting and provides authentic basis for the use of Torilis leptophylla in in vivo applications. In addition, the metabolic pool of phytochemicals can play a noteworthy role in drug discovery and development.

Keywords: Torilis leptophylla, metabolic profiling, HPLC-DAD-ESI analysis, C-glycosides