Cordia rufescens A. DC, a small shrub in northeastern Brazil is popularly referred to as "old-growth" or "pigeon-claw". This plant is used in folk medicine as abortive, anti-inflammatory agent and in the treatment of dysmenorrhea and dyspesia (VALE et al., 2012). Thus, the need for a greater chemical and pharmacological knowledge of Cordia rufescens A.DC species was observed, necessitating a more in-depth study of the isolation and characterization of new compounds. The plant material (leaves) was collected in 2002 in the municipality of Cruz do Espírito Santo - PB and identified by Profa. Dr. Maria de Fátima Agra. The material was then oven dried with circulating air at an average temperature of 45 ° C, ground in a mechanical mill and subjected to steeping with 95% EtOH. The crude ethanolic extract (106.10 g) was dissolved in a methanol: water (7: 3 v / v) solution and partitioned with the following solvents: hexane, dichloromethane and ethyl acetate. An aliquot of the dichloromethane phase (7 g) was subjected to the chromatographic column (CC), using as a stationary phase silica gel, and as mobile phase hexane, ethyl acetate and methanol, with elution systems in increasing polarity order, obtaining 34 fractions. All fractions were submitted to analytical thin layer chromatography (ADCC) and analyzed and assembled according to their retention factors (Rfs) and after visualization in ultraviolet light. Fractions 2, 3 and 5 presented different retention factors and therefore were submitted to liquid chromatography of high efficiency in analytical scale, where fraction 5 was chosen to obtain a better resolution of the chromatogram in front of the others, being then submitted to the purification by HPLC on a semi-preparative scale that resulted in the identified substances 4-hydroxy-benzaldeído and 4 -hydroxy-3,5-dimethoxycinnamate, reported for the first time in the species under study.