Terpenoids play a crucial role in the performance of diverse ecological functions in response to biotic and abiotic factors. They are classified as secondary metabolism products. Among their functions are: be pollinating attractants, herbivore deterrents, insecticides / repellents, antibacterial compounds. Like many other natural products, they show biological activities, which have been exploited in the prevention and treatment of human diseases. As part of the phytochemical study performed on the Cissus incisa specie, the characterization using GC/MS of a triterpenes mixture of the chloroform / methanol 1:1 extract was carried out. In addition, the antibacterial activity was evaluated against nine strains of multi-resistant clinical isolates. As a result, C. incisa can be considered for further investigations as a source of compounds with antibacterial properties