The human being since ancient times has sought in plants to cure their various diseases. The plants used for traditional medicine contain a wide range of substances that can be used to treat chronic and infectious diseases. Phytochemical researchers have great interest in the selection of medicinal plants for new therapies. In the present study, the bioactive components of the leaves of Cissus incisa have been identified using gas chromatography-mass spectrum (GC-MS). Sixteen compounds were identified in hexane extract. β-Sitosterol (19.445%) was the predominant compound in the hexane extract, which is a well-known biologically active compound. All compounds are reported for the first time in the species. The present investigation is the base for a later phytochemical study. With which it is possible to isolate different metabolites of biological interest.