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Molecular detection of virulence genes in Staphylococcus aureus associated with bovine mastitis
1  Universidad de la Ciénega del Estado de Michoacán, Genómica alimentaria, 58518, Sahuayo, Michoacán, México


Biofilm formation is considered a defense mechanism against environmental, chemical or biological factors. Some of the main bacteria with greater capacity for the formation of these structures are gram positive, in which Staphylococcus aureus stands out. This bacterium is implicated in a large number of diseases of a livestock nature, such as bovine mastitis, in which the ability to form a biofilm gives the bacteria a high resistance to chemical and antibiotic treatments, causing economic losses to increase. Therefore, the work seeks to identify in a molecular way the genes that are involved in the formation of biofilms, and to know if the bacteria possess one or more of said genes. The bacteria were isolated in the municipalities of Venustiano Carranza (AVC) and Marcos Castellanos (AMC), 39 and 15 respectively, for a total of 55 samples. Total DNA was extracted from all samples, its quality was verified by electrophoresis, to finally perform the genetic detection using endpoint PCR. A total of 10 genes were tested, of which their presence in bacteria with the ability to form biofilms was already proven. The ATCC isolate was used as a positive control. Only the icaD and icaA genes amplified, for a percentage of 27% and 24% respectively for the AMC isolates. In the case of AVC, the data was 41% for icaD and 37% for icaA. Comparing the data obtained with a work carried out by Toro Castillo in 2018, in which he sought the best biofilm formers with the same group of bacteria, it was found that bacteria with such capacity also had said ica genes, directly relating them to the formation of biofilms.

Keywords: Bioflims, genes, Staphylococcus aureus, mastitis