The present work was carried out on 133 halophilic strains, isolated on MGM medium at 12 and 23% (w / v) of salt. A screening of the extracellular proteolytic activities, carried out on the same medium supplemented with casein or gelatin at 1% (w / v), allowed us to select 24 bacterial strains and 21 Archean strains presenting a precipitate around the colonies for casein and / or a translucent halo (after addition of Frazier's reagent) for the gelatin. The enzymatic test was done on liquid medium in micro-culture on a 2 mL Eppendorf tube. The assay of the proteolytic activity using Azocasein as substrate, following 2 protocols the first with PBS and the second with tris HCl, with positive and negative controls, demonstrated interesting results for 10 strains among the 45 tested including 5 bacteria and 5 archaea. These have undergone morphological, physiological and molecular characterization based on amplification and sequencing of the 16S ribosomal RNA gene.