Aegopodium podagraria L. (goutweed) is a plant, widely grown in Europe and Asia. In folk medicine, mainly the leaves of goutweed were used to treat gout, inflammatory states in kidneys and bladder and to facilitate wound healing. Despite the presence of many pro-healthy compounds, e.g. polyacetylenes (falkarinol and falkaridiol), essential oils from the group of mono- and sesquiterpenes, polyphenols, e.g. phenolic acids and flavonoids, coumarin compounds, there is not much research on the properties of this plant. The aim of the study was to investigate the antioxidant properties of ethanolic and aqueous extracts of goutweed. The antioxidant effect of extracts from flowers, leaves, seeds and rhizomes of the plant was tested on the macrophages. The effect of extracts on the parameters of cytotoxicity (proliferation, apoptosis) were investigated to verify the safeness. The extracts protected cells against oxidative stress and had a positive impact on cell proliferation. Spectrophotometric methods demonstrated the high antioxidant capacity of extracts of flowers and seeds. In vitro studies on macrophages showed that the same extracts increase antioxidant potential and enzyme activity, which may inhibit free radical reaction by antioxidants contained in extract. Rhizomes and leaf extract initiated stress, increased enzyme activity and antioxidative potential as a defense mechanism. Extracts of seeds of goutweed added to the food or supplements can reduce oxidative stress, which may be used in the treatment and prevention of many diseases.