This paper aims to develop the vacuum electrospray deposition technique, which represents a gradual change in the range of scientific research through comparing between electrospray ion beam deposition and conventional deposition techniques. The electrospray deposition instrument is considered a simple method and has several advantages. Moreover, this paper presented an electrospray deposition source designed with and without mass selection used. A new phase which is called the deflection chamber stage has been added to the electrospray deposition source which is very similar to other electrospray deposition sources of the commercially available UHV-4 system from Molecularspray Ltd. This stage plays an important role by bending the ion beam in order to work towards separating two components of the beam. Just a few groups worldwide follow this method since the scale and price of the present mass-selected electrospray deposition systems are not permitted in designing an instrument that can be installed in several research laboratories. The number of molecules was deposited using this technique such as fluorescein, ferrocene, and a mixture of fluorescein and ferrocene. As well as, I included some data for spraying different solutions by presenting four experiments that have collected using the electrostatic ion deflection from spraying fluorescien (water + methanol 50:50), fluorescien dissolved in methanol, ferrocene dissolved in methanol, and a mixture of ferrocene and fluorescein dissolved in methanol. In my future work, I have planned to conduct SIMION software to model the system in an ion workbench scenario to simulate the energies and masses of the molecular ions through the system in order to refine the mass-selection process.