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STUDIES ON MCL-POLYHYDROXYALKANOATES USING DIFFERENT CARBON SOURCES FOR NEW BIOMEDICAL MATERIALS.
* 1, 2 , 3 , 1
1  University Politehnica of Bucharest, Faculty of Applied Chemistry and Materials Science, 1-7 Gh. Polizu Street, 01106, Bucharest, Romania
2  National Institute for Chemical Pharmaceutical Research and Development, 112 Vitan Ave, 031299, Bucharest, Romania
3  National Institute for Chemical Pharmaceutical Research and Development, 112 Vitan Ave, 031299, Bucharest, Romania

Abstract:

Polyhydroxyalkanoates (PHAs) are microbial homo- and copolymers of [R]-β-hydroxyalkanoic acids, produced by a wide variety of bacteria as an intracellular carbon and energy reserve. To obtain mcl-PHAs of microbial origin we used a Pseudomonas spp. strain (from the National Institute for Chemical-Pharmaceutical Research and Development (ICCF) culture collection of micro-organisms), by varying the carbon sources and the precursors.

In this work, assays were performed with fermentation media seeded with inoculum cultures of strain Pseudomonas putida in a proportion of 10%.

The influence on mcl -PHA production of carbon sources for strain development, hexanoic (C6), heptanoic (C7), octanoic (C8) and nonanoic (C9) acids, as polymers precursors, were analyzed. Due to their properties, similar to those of conventional plastics and to their biodegradability, PHAs are suitable for many applications and for biomedical materials useful in surgical sutures, tissue engineering and drugs carriers, which leads us to the deepening of the study of obtaining micro/nanofibers by the electrospinning method.

Keywords: polyhydroxyalkanoates; bioprocess; biomaterials; electrospinning;
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