Physical exercise is recommended to decrease bone loss in elderly women. Supejump® is a new workout activity performed on a modified elastic mini trampoline that mixes aerobic and anaerobic exercises . The aim of the study was to investigate the long-term effects of training with Supejump® on bone metabolism in women. Twelve women were involved in the study. They performed Supejump®, three times a week, each session lasting 60 mins for a total of 20-weeks. Blood samples were collected at baseline and at the end of the 20-weeks of training. The biomarkers of bone resorption (c-terminal telopeptide region of collagen type 1 (β‐CTX)), bone formation (osteocalcin), and calcitonin, parathyroid hormone (PTH), calcium, phosphate, cortisol, total vitamin D, were analyzed. The β-CTX and PTH were significantly reduced after 20 weeks of Supejump® training while calcitonin, calcium and total vitamin D were increased. These data suggest that the 20-weeks intervention had a high impact in reducing bone resorption and improving bone metabolism. In conclusion Supejump® training may be considered as a valuable intervention to prevent osteoporosis in aging because it improves bone metabolism.