Plants are exposed to continuous environmental challenges and depending on the intensity and time of exposition to a specific environmental condition, plants either overcome the adverse situation or undergo a different level of injuries such as nitro-oxidative damages of biomolecules [1,2]. Xanthine oxidoreductase (XOR) is a FAD-, molybdenum-, iron- and sulfur-containing hydroxylase enzyme involved in the purine catabolism pathway that catalyzes the conversion of hypoxanthine/xanthine to uric acid with the concomitant formation of either NADH or superoxide radical (O₂^•−) which is then dismutated into H₂O₂. In fact, under environmental stress conditions, XOR is considered to participate in the generation of reactive oxygen species (ROS) [3,4]. Using pea (Pisum sativum L.) plants exposed to six different environmental conditions including high light intensity, low and high temperature, continuous light, continuous dark and mechanical wounding, XOD was analyzed at protein and gene expression levels. The obtained data indicate that XOD is modulated differentially under the assayed stress conditions being the low temperature the situation with causes the highest differences of XOD at gene and protein expression.

[1] Corpas et al., 2008. Plant Cell Physiol. 49, 1711-1722.
[2] Corpas et al. 2009, J Plant Physiol. 165, 1319-1330.
[3] Zarepour et al. (2010) Plant Mol. Biol. 72: 301-310.
[4] Ma et al. (2016) Plant Cell. 28, 1108-1126.

[Supported by grants PID2019-103924GB-I00 and P18-FR-1359 from MICINN and PAIDI-Junta de Andalucía, Spain]