Please login first

Use of molecular dynamics to decipher the binding of salicylic acid to proteins: Example of Arabidopsis thaliana Chloroplastic GAPDH-A1
Igor Pokotylo 1 , Denis Hellal 1 , Tahar Bouceba 2 , Volodymyr Kravets 3 , Miguel Hernandez-Martinez 1 , Isabelle Kleiner 4 , Eric Ruelland * 1
1  IEES-Paris (UMR_7618) - Institut d’écologie et des sciences de l’environnement de Paris; CNRS UMR 7583, Université Paris-Est Créteil, Sorbonne Université, F-94010 Paris ; France
2  Plateforme d’interactions moléculaires, CNRS-FR3631; Institut de Biologie Paris Seine (IBPS), Sorbonne Université, F-75252 Paris Cedex 05, France
3  V.P. Kukhar Institute of Bioorganic Chemistry and Petrochemistry, National Academy of Sciences of Ukraine, 02094 Kyiv, Ukraine
4  LISA (UMR 7583) - Laboratoire Interuniversitaire des Systèmes Atmosphériques (LISA), CNRS UMR 7583, Université Paris-Est Créteil, Université de Paris, Institut Pierre Simon Laplace (IPSL), 61 avenue du Générale de Gaulle, F-94010 Créteil, France


Salicylic acid (SA) has an essential role in the responses of plants to pathogens. SA initiates defense signaling cascades via binding to proteins. NPR1 is a transcriptional co-activator and is a key target of SA binding. Many other proteins have been shown to bind SA. Amongst these proteins are important enzymes of primary metabolism. Here, we detail that the A1 isomer of chloroplast glyceraldehyde 3-phosphate dehydrogenase (GAPA1) from Arabidopsis thaliana binds SA, as shown in surface plasmon resonance experiments. Besides, we show that SA inhibits its GAPDH activity in vitro. To gain some insight into the underlying molecular interactions and binding mechanism, we combined in silico molecular docking experiments and molecular dynamics simulations on the free protein and protein–ligand complex. The molecular docking analysis yielded to the identification of two putative binding pockets for SA. A simulation in water of the complex between SA and the protein allowed us to determine that only one pocket—a surface cavity around Asn35—would efficiently bind SA in the presence of solvent. The importance of this is further supported through experimental biochemical assays. Indeed, mutating GAPA1 Asn35 into Gly or Arg81 into Leu strongly diminished the ability of the enzyme to bind SA. The very same cavity is responsible for the NADP+ binding to GAPA1. NADH inhibited, in a dose-response manner, the binding of SA to GAPA1, validating our data. The use of the methodology to study SA binding to other proteins will be discussed at the end of the talk.

Keywords: salicylic acid; glyceraldehyde 3-phosphate dehydrogenase; molecular dynamics; molecular docking; protein ligand interaction; surface plasmon resonance; biacore