Biocides disinfectants are used in agro-food industries in order to limit the development of pathogens present in environment or on surfaces in contact with food for human or animal consumption. Biocides residues remaining on food surfaces may constitute a toxicological risk for the consumer. Very little feedback from the field on the rates of biocides residues in the industry exist due to a lack of simple, fast and responsive self-checking methods. The development of biosensors for the detection of biocides disinfectants represent a promising way to explore but most of this research remains limited.