As of March 2021, the Family: Picornaviridae contained 158 species grouped into 68 different genera. We report the identification of a new Picornaviridae-like viral species isolated from the Wild Lime Psyllid (WLP), Leuronota fagarae. Extraction and sequencing of nucleic acid from WLP adult salivary glands identified a 5554 nt sequence with 52.75% identity to Diaphorina citri (Asian Citrus Psyllid) picorna-like virus polyprotein and 59.61% identity to Bemisia tabaci (Silverleaf Whitefly) polyprotein, NCBI BlastX and Blastp analysis. Sequence comparisons of amino acids and nucleotides showed consistent similarity and motifs with picorna-like virus polyproteins across 8 known species, having significant E-values of 7e-116 or less. Polyproteins are around 2100-2400 in length, are cleaved into multiple active peptides to allow for viral replication. Phylogenetic comparisons using amino acid and nucleic acid polyprotein sequences shows a diverse radiant group of insect hosts. The discovery of a picorna-like virus in WLP that is related to the D. citri Picorna-like virus, whose niches overlap, may provide new insight into management of psyllid vectors that transmit crop pathogens, like the bacterium causing Huanglongbing in citrus.
Previous Article in event
Next Article in event Next Article in session
Picorna-Like Virus Discovered in Wild Lime Psyllid Leuronota fagarae Burckhardt (Hemiptera: Psylloidea)
Published: 30 June 2021 by MDPI in The 1st International Electronic Conference on Entomology session Genetics and Genomics
Keywords: Huanglongbing; Picornaviridae; Diaphorina citri, Iflaviridae; polyprotein, RdRp, SNPs