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Amide I band analysis applied to vibrational micro-spectroscopies of gingival crevicular fluid samples for orthodontic treatment monitoring.
1 , 2 , 3 , 2 , 3 , 4 , * 5
1  CNR-SPIN, Istituto Superconduttori, Materiali Innovativi e Dispositivi, via Campi Flegrei 34, Pozzuoli 80078, Italy
2  Multidisciplinary Department of Medical-Surgical and Dental Specialties, University of Campania Luigi Vanvitelli, 80138 Napoli, Italy
3  Dip. Multidisciplinare di Specialità Medico-Chirurgiche e Odontoiatriche, Università degli Studi della Campania Luigi Vanvitelli, via L. De Crecchio 6, Napoli 80138, Italy
4  Dipartimento di Medicina Sperimentale, Università della Campania "Luigi Vanvitelli", Napoli
5  Dip. di Medicina Sperimentale, Università degli Studi della Campania Luigi Vanvitelli, via S. Maria di Costantinopoli 16, Napoli 80138, Italy
Academic Editor: Stefano Mariani (registering DOI)

Vibrational micro-spectroscopies have been applied to investigate gingival crevicular fluid (GCF) for monitoring the orthodontic treatment with fixed appliances [1-3]. In particular, GCF samples have been investigated using Fourier transform infrared, Raman, and Surface-Enhanced Raman micro-spectroscopies. GCF samples were collected from patients aged between 12 and 22 years before bracket bonding and after 2, 7, and 14 days of treatment at the buccal side of the incisors. The GCF spectra collected at different times of orthodontic tooth movement have been used for characterizing biochemical changes occurring during the treatment. The examined spectra show that Amide I band due to protein contents give relevant information.

In the present work, we examined the Amide I band region with particular attention by using deconvolution analysis using Gaussian-Lorentzian curves for infrared spectra and Lorentzian curves for Raman spectra. The performance of the procedure was evaluated using the χ2 parameter.

The deconvolution analysis allowed us to evidence the contribution of the different subcomponents of the Amide I band and the changes occurring during orthodontic treatment. These changes can be ascribed to modifications in the secondary structure of protein content and can contribute to make vibrational spectroscopies a useful tool for monitoring the individual patient’s response to the orthodontic force application.


[1] F. d’ Apuzzo, L. Perillo, I. Delfino, M. Portaccio, M. Lepore, C. Camerlingo Monitoring early phases of orthodontic treatment by means of Raman spectroscopies Journal of Biomedical Optics 22(11), 115001 (2017), DOI: 10.1117/1.JBO.22.11.115001.

[2] M. Portaccio, F. d’ Apuzzo, L. Perillo, V. Grassia, S. Errico, M. Lepore Infrared microspectroscopy characterization of gingival crevicular fluid during orthodontic treatment Journal of Molecular Structure 1176 (2019) 847-854

[3] F. d' Apuzzo, L. Nucci, I. Delfino, M. Portaccio, G. Minervini, G. Isola, I. Serino, C. Camerlingo, M. Lepore “Application of vibrational spectroscopies in the qualitative analysis of gingival crevicular fluid and periodontal ligament during orthodontic tooth movement” J. Clin. Med. 2021, 10, 1405.

Keywords: Amide I; band deconvolution; Raman spectroscopies; infrared spectroscopies; orthodontic tooth movement; gingival crevicular fluid