Oat (Avena L.) is an important animal feed and food crop. It belongs to alternative cereals, which can properly supplement traditional types of cereals. The goal of the study was to analyze the genetic variability of oat genotypes using SCoT technique, which uses gene specific primers. In the study, there were analyzed a total of 22 varieties of oats, of which 20 varieties were wild oats, and 2 of them were naked oats, that come from different European countries and Canada, using 7 SCoT markers. In total, 40 fragments were amplified of which 26 were polymorphic with an average number of 3.71 polymorphic fragments per genotype. Using SCoT8 marker the highest number of polymorphic fragments (5) was detected. The highest percentage of polymorphism revealed SCoT23 marker (80 %). The average percentage of polymorphism was 65.67 %. The value of polymorphic information content (PIC), which proves the ability of the marker to differentiate genotypes, ranged from 0.305 (SCoT12) to 0.674 (SCoT8) with an average value of 0.506. In the dendrogram constructed using hierarchical cluster analysis the genotypes of oats were divided into two main groups whereby these groups were divided into several subgroups. Based on analyses two genetically the closest genotypes, cultivar Alf (Germany) and Amursky utes (Russia), grouped in the subgroup Ia. Two naked oats (Avena nuda L.), Czech genotype Izak and Slovak genotype Hronec, grouped side by side in subcluster Ia. Used SCoT markers showed the ability to identify and differentiate genotypes of the common and naked oat.