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Development of method for determination of diacerein in raw material by HPLC
1  Hanoi University of Pharmacy, Vietnam
Academic Editor: Humbert G. Díaz

Abstract:

An HPLC-DAD method was developed and validated for determination of diacerein in raw material. The chromatographic conditions are as follows: Column: Luna RP-C18 (4,6 mm x 250 mm; 5 µm). Detector UV, λ = 272 nm. Mobile phase: Buffer solution : Acetonitrilee (55 : 45), (Buffer solution: Mix 19.6 mL of 0.1 mol/L Citric acid and 0.4 mL of 0.2 mol/L sodium monohydrogen phosphate); Flow rate: 1.5 ml/min. Temperature: room temperature.

Keywords: HPLC-DAD; diacerein, acetonitrile, N, N-Dimethylacetamide, valiadation, determination
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