Increasing the tolerance and resistance of pathogens to conventional antibiotics is a global health issue and there is a need to use effective and new substances. MOFs are highly functional materials with antimicrobial properties come from their composition, structure, and high internal volume, which could be a source for antimicrobial guest molecules integrated in the pore. In addition, MOF can contain more than one type of metal ion in the same structure. In this work, a metal-organic framework, [Ba(H₂btec).H₂O]_n was synthesized by the deposition method using benzene-1,2,4,5-tetracarboxylic acid (H₄btec) and Ba(NO₃)₂. Characterization of MOF was performed using XRD, XRF, FTIR, SEM analyses. The metal-organic framework used against gram-positive and gram-negative bacteria including Keleb peneumonia, Staph coccus aureus, Staph sapropphyticus, Esherichia coli.