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Functionalization of Graphene Oxide for Label-free Electrochemical Detection of Hepatic Cancer Cells
* 1 , 2 , 3 , 4
1  Department of Chemistry, College of Sciences, University of Sharjah, Sharjah, UAE
2  Wolfson Nanomaterials and Devices Laboratory, School of Engineering, Computing and Mathematics, Faculty of Science and Engineering, University of Plymouth, Plymouth, UK
3  Zimmer and Peacock Ltd., Royston SG8 9JL, UK
4  Institute of Synthetic Bioarchitecture, Department of Bionanosciences, University of Natural Resources and Life Sciences, 1190 Vienna, Austria
Academic Editor: Giovanna Marrazza


In this study, a graphene oxide (GO) modified screen-printed carbon electrode (SPE) was functionalized with 1-pyrenebutyric acid-N-hydroxy-succinimide ester (PANHS) and conjugated with antibodies for the label-free detection of human hepatoma HepG2 cells (Fig. 1). Using a user-friendly reservoir chamber, the functionalized film was exposed continuously to the cancer cells. The continuous flow is intended to enhance capture of the target cells onto the sensing platform. The response of the biosensor was evaluated using cyclic voltammetry (CV). The preliminary data showed good sensitivity for detection of hepatic cancer cells. The developed biosensor could detect the HepG2 cells from 1 × 103 to 3 × 105 cells/mL range. Thus, it provides a simple tool for electrochemical detection of cancer cells and offers low-cost and disposable sensing platform.

Figure 1. Schematic illustration of the developed electrochemical biosensor for detection of cancer cells (not drawn to scale).

Keywords: Electrochemical biosensor; Flow cell; Graphene Oxide; Linker