Helicobacter pylori (H. pylori) is a gram-negative, slow-growing bacterium, microaerophilic, and a unique gastric pathogen causing chronic inflammation in the gastric mucosa with a possibility of developing gastric cancer has one-third of its proteins still uncharacterized. In this study, computational analysis has been done on the structural, functional, and epitopic characteristics of a hypothetical protein (HP) from H. pylori named HPF63-1454. The model prediction and primary, secondary, and three-dimensional structures of the chosen HP were built. The newly created model appeared to have high quality once refinement and structure validation was completed. The anticipated tertiary structure was assessed using the Swiss Model assessment. The best materials are chosen using structural analyses considering data from the Z scores, Swiss Interactive Workplace, and Ramachandran plot statistics. This investigation aimed to determine the importance of the H. pylori protein HPF63-1454. Therefore, this research will increase our understanding of pathophysiology and allow us to target the protein complex specifically. H. pylori infections are now treated with readily available antibiotics and acid-suppressing medications, but the species soon established itself as a hardy pest. Long-term and taxing antibiotic therapy for H. pylori infection is increasingly beginning to fail, necessitating novel, creative therapeutic modalities.