The beneficial health effects of pomegranate have consistently garnered scientific interest. Pomegranate, including its juice, seeds, and seed oil, has been recognized for its remarkable nutraceutical properties. This study focuses on the investigation of pomegranate seed oil obtained from the Beni-Snouss region in northwest Algeria.

Various Soxhlet extractions were performed to obtain the seed oil, resulting in yields ranging from 11% to 17%, depending on the choice of solvent. The quality of the oil was evaluated using several parameters, including the Iodine index, Acid index, and Saponification index. High Performance Thin Layer Chromatography (HPTLC) and Gas Chromatography with Flame Ionization Detection (GPC-FID) were employed for the identification and quantification of fatty acids and glycerides.

Our results revealed the exclusive presence of triglycerides, with the absence of phospholipids. Notably, the fatty acids were predominantly unsaturated (comprising 95% of the total), particularly polyunsaturated fatty acids, accounting for over 89%. Of particular significance, an exceptionally high concentration of punicic acid (83.20%) was detected, establishing our pomegranate seed oil as one of the richest sources of punicic acid worldwide.

This research highlights the potential of pomegranate seed oil from Beni-Snouss as a valuable nutraceutical resource, particularly due to its exceptional punicic acid content.