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DNA fluorescence probes based on N-[5-(11-functionalised-undecylamino)- 9H-benzo[a]phenoxazin-9-ylidene]propan-1-aminium chlorides
* 1 , 2 , 2
1  Centro de Física, Universidade do Minho, Gualtar, 4710-057 Braga, Portugal
2  Centro de Química, Universidade do Minho, Gualtar, 4710-057 Braga, Portugal

Abstract: Several fluorescent benzo[a]phenoxazinium chlorides possessing undecylamino chains with non-functionalised (methyl group) or functionalised terminals (hydroxyl, carboxylic acid and the ester group) as substituents at the 5-position of the heterocycles were used in photophysical studies with DNA. It was found that the functionalised terminal has a dramatic influence on the type of interaction with the hydroxyl group promoting intercalation, while the ester group promotes groove binding.
Keywords: Benzo[a]phenoxazinium dyes; Nile Blue; DNA probes; Near-Infrared fluorophores