In bamboos, leaves are central not only to plant physiology but also to taxonomic study, since macromorphological characteristics such as culm or branching often overlap among species. Leaf anatomy therefore provides a valuable lens for understanding how bamboo functions and for resolving its often-challenging classification. This study focused on finding out the anatomical differences among the leaves of four bamboo species, Bambusa longispiculata, Bambusa ventricosa, Bambusa multiplex, and Dendrocalamus giganteus. The plant leaves were collected from Botanical Garden of Rajshahi University, Rajshahi, Bangladesh. The surface of the leaf blade was carefully peeled, and stomatal characteristics were observed microscopically. For microscopic structural analysis, slides were prepared from the leaf blade section. The radial extent of the cell was measured utilizing image J softwere. The leaf length, width, and area significantly varied within the bamboo species studied. The stomatal index in B. ventricosa was the highest in percentage (17.39%) and lowest in the species D. giganteus (6.9%). A variation was found in the arrangement and layer of the epidermal cell between the costal and intercoastal zones. In the transverse section, the largest diameter was found in the fusoid cell (147.36±23.19 um) in the midst of other cells, i.e., the Meta xylem, proto xylem, phloem, mesotome, bundle sheath, mesophyll, and bulliform. Among the species, B. longispiculata had the largest cell diameter and B. multiplex had the smallest cell diameter. Variations were found in the number and arrangement of vascular bundles, as well as in the diameters of the mesophyll and bundle sheath cells, as well as other cells among the studied species. These findings demonstrate the importance of anatomical study in understanding bamboo species' physiology, ecological strategies, and functional adaptations. The findings also undercore the interspecific variation among the species.