miR-200c and PRR11: A Diagnostic Duo in HPV-Driven Cervical Cancer&mdash;Unraveling Their Inverse Regulation for Early Detection

Kaoutar ANOUAR TADLAOUI

Previous Article in event

Brain Cancer Gene Expression Analysis for Subtype Classification and Biomarker Discovery Using Machine Learning

Next Article in event

Rational Design of SIRT2 Inhibitors Using Combined 3D-QSAR, Docking, and Machine Learning Methods

Next Article in session

A Conceptual Nanotheranostic Platform Using Functionalized Metal Quantum Dots for Targeted Cancer Imaging and Therapy

miR-200c and PRR11: A Diagnostic Duo in HPV-Driven Cervical Cancer—Unraveling Their Inverse Regulation for Early Detection

Kaoutar ANOUAR TADLAOUI

¹ Laboratory of Molecular Biology, Higher School of Biomedical Engineering and Health Technology SUPTECH SANTE - Mohammedia, Morocco.
² Research team of Virology, Oncology and Biotechnologies. Laboratory of Virology, Oncology, Biosciences, Environment and New Energies. Faculty of Sciences and Techniques Mohammedia, University Hassan II of Casablanca, Morocco.

Academic Editor: Masaharu Seno

Published: 05 June 2026 by MDPI in The 5th International Electronic Conference on Cancers session Causes, Diagnosis and Treatment of Cancer

Abstract:

Introduction: The persistent infection of Human Papillomavirus (HPV) is one of the most significant risk factors for the development of cervical cancer (CC). The complexity of the disease process involves multiple factors that are not completely understood. Recent studies show that in cervical cancers associated with HPV infections, the tumor suppressor miR-200c is downregulated, whereas proline-rich protein 11 (PRR11), which stimulates oncogenesis, is upregulated. Nonetheless, the potential of miR-200c and PRR11 to be in an inverse regulatory relationship in Moroccan patients suffering from CC and the possibility of using them in combination as cancer diagnostic tools remains unexplored.

Objectives: The objective of this study is to examine the expression profiles of miR-200c, PRR11 and HPV in CC biopsies and review the potential of these genes and virus as diagnostic markers for HPV-positive cervical cancer.

Materials and Methods: The expression levels of miR-200c, PRR11, and the HPV virus were quantified using RT-PCR in 200 cervical biopsies (100 malignant biopsies and 100 healthy tissues). The statistical significant correlations, as well as the estimation of diagnostic potential, were performed using Jamovi statistical software.

Outcome: There was notable identification of reverse correlation of miR-200c and PRR11 in relation to SCC with HPV. Cancer tissue samples showed significant upregulation of PRR11 and downregulation of miR-200c when compared to samples from healthy individuals. HPV related miR-200 dysregulation and PRR11 was found to be significant in relation to the disease process (p < 0.05). For the purpose of diagnosis, the evaluation of miR-200c and PRR11 was the strongest with 97.85 % sensitivity, 89.67 % specificity and AUC of 0,910 which supports the potential for diagnosing cervical cancer.

Summary: The upregulation of PRR11 and the downregulation of miR-200c provides the basis of the etiology for CC HPV-induced. The evaluation of PRR11 and miR200c jointly provides an opportunity for early diagnosis.

Keywords: PRR11, miR-200c, HPV, Cervical Cancer, Diagnosis, Biomarker.

0 Reads
0 Recommendations

Kaoutar ANOUAR TADLAOUI