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In vitro antidiabetic evaluation guided by GC-MS analysis of ethyl acetate leaves extract of Acacia auriculiformis Benth.
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1  Department of Pharmaceutical Sciences & Technology, Birla Institute of Technology, Mesra, Ranchi, Jharkhand, India-835215

Published: 31 October 2018 by MDPI in 4th International Electronic Conference on Medicinal Chemistry session ECMC-4

Acacia auriculiformis Benth. is an evergreen shrub traditionally used as a remedy in the cure of itching, aches, allergy, rheumatism, and sore eyes. But recently, this shrub has been explored for antidiabetic activity. Here in this work, we have attempted to evaluate various leaves extracts of Acacia auriculiformis Benth. for α-glucosidase assay and α-amylase assay and thereafter the bioactive ethyl acetate leaves extract was subjected to GC-MS analysis. The in vitro antidiabetic evaluation showed promising results. The % inhibition of ethyl acetate leaves extract in the α-glucosidase assay and the α-amylase assay was found be 94.259% and 95.259% respectively, at a concentration of 10 μg/ml. The LC-MS results revealed the presence of phenolic compounds (phenol, 2,4-bis(1,1-dimethylethyl); 2,4-Ditert-butylphenol) and various esters (hexadecanoic acid, methyl ester; 1,2-benzenedicarboxylic acid, butyl octyl ester; hexadecanoic acid, 2,3 bis[(trimethylsilyl)oxy]propyl ester). Those compounds could be responsible for the desired antidiabetic activity.

Keywords: Acacia auriculiformis Benth.; Antidiabetic activity; α-Glucosidase Assay; α-Amylase Assay; GC-MS