Water pollution represents one of the main consequences of anthropogenic activity, therefore, recent research has developed promising techniques for treating wastewater through the use of natural coagulants-flocculants, which generate higher water quality and product reduction environmentally harmful synthetics and human health. Moringa oleifera seeds are reported as the most potent natural coagulants, and according to the growth of the tree in different landscapes and gradients, they present variability in their biological function. The objective of the work was to evaluate the efficiency of M. oleifera seeds collected in urban forests of the city of Ibagué, Colombia as a natural coagulant-flocculant in the treatment of water for human consumption. Seeds were collected ad libitum from trees present in forest remnants inside the city, dried, pulverized and subjected to the process of extraction of the active agents from organic solvents; samples were taken from river surface waters and with a latin square design (DCL) that grouped two factors (water pH and extract dosage), the coagulant action was evaluated in the tests carried out with jar tests. According to the ranges of pH (4, 5, 6 and 7) and dosages (5, 10, 15 and 20 ml) used, a greater reduction of the turbidity values ​​was obtained in the conditions of pH 6 with a dosage of 10 ml of coagulant solution, and pH 7 with a dosage of 10 ml of coagulant solution. The tests carried out showed that the coagulation-flocculation levels of moringa seeds do not vary significantly concerning the reports in the literature for Colombia, their performance is associated with the factors established in the experimental design and maintains experimental efficiency against coagulants conventionals used for the purification of water.