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The Mineral, Amino Acid and Fatty Acid Evaluations of Myristica fragrans Seeds Extracts
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1  Department of Biochemistry, University of Nigeria, Nsukka


The mineral elements, amino acid constituents and fatty acids present in Myristica fragrans seeds extracts were identified and quantified using atomic absorption spectrometry and flame photometry, Technicon sequential multi-sample amino acid analyzer (TSM) and Shimadzu GC-MS machine respectively. Six hundred grams of powdered seeds were extracted in methanol and n-hexane (2:1 v/v) using a rotary evaporator. Calcium, magnesium, and iron were the most abundant in methanol extract with concentrations of 3.207 ± 0.048 part per million (ppm), 2.998 ± 0.046 ppm, and 0.977 ± 0.022 ppm respectively. The major essential amino acids in the seed were leucine 6.24 g/100g protein, valine 3.72 g/100g protein, and threonine 3.50 g/100g protein while the non-essential amino acids were glutamate 10.6 g/100g protein, aspartate 7.60 g/100g protein, and arginine 5.50 g/100g protein. The major biological compounds in the methanol extract as revealed by the GC-MS analysis were 9,12-Octadecadienoic acid methyl ester (RT: 20.768, 27.13%), Cyclododecyne (RT: 26.458, 19.33%) and octadecanoic acid (RT: 14.360, 12.24%) while the hexane extract constituents were margarinic acid (RT: 14.746, 27.04%), oleic acid (RT: 20.947, 18.96%) and 9,12-octadecadien-1-ol (RT: 26.523%, 15.10%). These compounds have shown nutritional, pharmacological, and medicinal importance in different studies.

Keywords: Mineral elements; Calcium;Myristica fragrans; Amino acids; Fatty acids