Rhizobia, known as legume endosymbionts, can serve as beneficial associative bacteria for many non-legume crops, including tomato. There are a number of studies to improve this associative interaction by transforming plants with various genes, in particular, the genes of plant lectins and bacterial agglutinins. It is known that glutathione (GSH) plays an important role in the formation of associative symbiosis between plants and bacteria. The aim of this work was to study the redox state of glutathione in various artificial symbiotic systems on the roots of tomato plants, transformed with the lectin genepsl and the bacterial agglutinin gene rapA1after treatment of R. leguminosarum strain. It was found that in the roots of wild-type tomato, treatment with the bacteria caused the accumulation of GSH, without affecting its oxidized form-GSSG and the indicator of the GSH/GSSG ratio was 3.2. Evaluation of the glutathione content in the roots of transgenic plants withpsl gene showed a significant increase in the GSH content and the GSH/GSSG ratio was 4.6, while the adsorption of bacteria on the roots of these plants increased 9 times. In the transgenic roots with the rapA1 gene bacteria were sorbed three times more in comparison with the control plants, and the GSH/GSSG ratio was 4.1. It should be noted that in both variants of the studied transgenic plants, bacteria did not affect the GSSG content and its value remained at the level of untreated plants, which indicates the absence of a negative effect of rhizobia on tomatoes. The results obtained demonstrate the promise of studying the redox state of glutathione for assessing the effectiveness of artificial symbiotic systems.