In the present work Cinnamomum zeylanicum gender a wildly used as spice and medicinal plant, is investigated for both chemical and biological field, since development of bio-guided trials on medicinal plants therapeutic target has increased recent years through pharmacology which is interested in discovering new molecules expressing a therapeutic activity and development of useful drugs by selecting the most active fraction and isolates the active compound responsible of the therapeutically effect.

Therefore, for studied spice, we performed identification and quantification analysis of main bioactive compounds in order to subject it to bio guided tests using several solvents' polarities in order to evaluate its in vitro antimicrobial potential. To achieve our objective, we used qualitative and quantitative methods to identify the components of the obtained extracts. The in vitro antimicrobial effect of the medicinal plant was evaluated by disk diffusion method on several ATCC microbial strains and which gave almost very interesting results for all tested pathogens in addition to a richness in secondary metabolites.