Abstract: Two new disazo compounds containing 4,4'-diaminostilbene- 2,2'-disulfonic acid and 4,4'-diaminobenzanilide as middle components were synthesized and characterized by thin layer chromatography (TLC), electronic spectra (VIS), FT/IR and 13 C-NMR spectroscopy. According to the results of the colouristic evaluation, the studied azo compounds can be ranged in the direct dye's class. The individual human skin tolerance for these azo compounds was evaluated by in vivo imagistic skin studies and the obtained results indicate that, from dermatologic point of view, the azo compounds may be used as textile dyes. Further, the compounds were evaluated for antibacterial activity against Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli, Pseudomonas aeruginosa and Proteus vulgaris by the disk diffusion method. The screening data releaved that the studied azo compounds exhibited antimicrobial activity against most of the tested species, presenting a particular interest against Staphylococcus aureus and Escheria coli.