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Reductive Biotransformation of Ethyl Acetoacetate: A Comparative Studies using Free and Immobilized Whole Yeast Cells
* 1 , * 1 , 2 , 3 , * 1
1  Biotransformation Lab, Division of Medicinal Chemistry, SVBs College of Pharmacy, MIDC phase II, Sonarpada, Dombivli (E), Dist: Thane 421203, India
2  Department of Biotechnology, Y. T. Institute of Pharmacy, Karjat, Dist: Raigad, India
3  Department of Pharmaceutical Microbiology, Satara College of Pharmacy, Satara-415004, India

Abstract: Bioreduction of ethyl acetoacetate with free and immobilized yeast whole cell was achieved by using water and sucrose combination. After detachment from immobilized beads under basic condition, the corresponding ethyl(S)-(+)-3-hydroxybutanoate was isolated with 98 to 100% yield. Immobilized beads of yeast whole cell were prepared at different temperature which affects the morphology and physiology of the beads for the diffusion of the enzyme, which shown the maximum conversion of the products as compared to the free yeast whole cell.
Keywords: Biotransformation; Ethyl acetoacetate; Ethyl(S)-(+)-3-hydroxybutanoate; Immobilized yeast whole cell; Free yeast whole cell