In this paper we reported the synthesis of new hydroxamic acids, containing heteroaromatic fragment of acridine-9(10H)-one. These compounds were prepared by interaction of corresponding carboxylic acid with hydroxylamine hydrochloride in the presence of CDI in DMF solution. The structure of obtained compounds was confirmed by IR, Mass-spectrometry, 1 H and 13 C NMR analyzed methods. Obtained compounds were screened for their in vitro antimicrobial activity against five bacterial and one fungal pathogens. Some synthesized compounds showed high efficiency against gram-positive strain Bacillus subtilis and gram-negative strain Proteus vulgaris.