Please login first
3D scaffolds via Multi-Photon Polymerization as a co-culture system for application in peripheral nervous system regeneration.
* 1, 2 , 1 , 1 , 1
1  Institute of Electronic Structure and Laser, Foundation for Research and Technology-Hellas (FORTH-IESL), 100 Nikolaou Plastira Street, Heraklion, 70013, Greece
2  Department of Materials Science and Technology, University of Crete, Vassilika Vouton, Heraklion, 71409, Greece
Academic Editor: VIJAYAKUMAR ANAND

Published: 13 March 2023 by MDPI in Holography Meets Advanced Manufacturing session Advanced Manufacturing posters
https://doi.org/10.3390/HMAM2-14148 (registering DOI)
Abstract:

Multi-Photon Polymerization (MPP), has found application in the field of Tissue Engineering (TE), due to the ability of fabrication of high precision scaffolds that can be used as a cell culture substrate. Of great importance is the Peripheral Nervous System (PNS) Tissue Engineering and Regeneration which shows increasing potential as an alternative to established methods, namely surgery and grafts, that aim to counter PNS-related diseases. A femtosecond fiber laser operating at 780nm (pulse duration:120fs, repetition rate:80MHz) was utilized to fabricate a novel pyramid-shaped scaffold geometry (400μm×400μm×60μm) using an organic/inorganic hybrid material. The scaffolds were used as a substrate for the mono- and co-culture of murine neuronal N2a and glial Schwann (SW10) cells for 7, 14 and 21 days with flat glasses as controls. Comparison between scaffolds and controls revealed cell and neurite directionality that was highly influenced by the presence of scaffold topography vs the random orientation controls exhibited, due to the cell responses to the topographical cues provided. In addition, the co-culture system provided a favorable environment for longer neurite formation after 21 days compared to mono-cultures, showing a 2-fold increase in neurites longer than 40μm (31.4%±5.5% vs 15.4%±5.4% of total neurites respectively), indicating a possible synergistic effect of co-cultures and scaffold topography. These findings suggest the ability to control neurite length and directionality, which are crucial parameters in PNS TE, and could form the basis for the development of an in vitro model for the study of PNS-related diseases.

Keywords: Multi-photon polymerization (MPP); Tissue regeneration; Peripheral Nervous System (PNS); Co-culture system; Scaffold Topography; Cell Orientation; Neurite Directionality
Comments on this paper
Miranda Joye
Damage to the Peripheral Nervous System (PNS) has been linked to a variety of neurodegenerative illnesses, attracting the attention of Tissue Engineering (TE), uno online which is viewed as a promising alternative to well known procedures such as surgery and autografts.
Alice Bobby
Interesting articles keep posting more! retro bowl college

james abel
The study revealed that the presence of the scaffold topography significantly drift hunters influenced the directionality of cell and neurite growth compared to the random orientation observed in the control group.

helen dam
Thank you for providing the abstract. It presents valuable information about the application of Multi-Photon Polymerization (MPP) in Tissue Engineering (TE), specifically focusing on sinister squidward the Peripheral Nervous System (PNS) Tissue Engineering and Regeneration.

sofia cole
We explore super-resolution computational diffraction in the inverse retrieval process. The optical construction of the lens uses a local light aperture time coding modulator, How Long Does It Take to Ghostwrite a Book?

orbor neee
The scaffolds were used as a substrate for the mono- and co-culture of murine neuronal N2a and glial Schwann (SW10) cells for 7, 14 and 21 days with flat glasses as controls. Comparison between scaffolds and controls revealed cell and neurite directionality that was highly influenced by the presence of scaffold topography vs the random orientation controls exhibited, due to the cell responses to the topographical cues provided. Super Mario

orbor neee
The scaffolds were used as a substrate for the mono- and co-culture of murine neuronal N2a and glial Schwann (SW10) cells for 7, 14 and 21 days with flat glasses as controls. Comparison between scaffolds and controls revealed cell and neurite directionality that was highly influenced by the presence of scaffold topography vs the random orientation controls exhibited, due to the cell responses to the topographical cues provided. Super Mario

Byrd Franklin
Good article. Very informative, well balanced, unbiased. x trench run

Louis Morton
Greetings, fellow adventurers! As someone who cherishes the thrill of exploration and discovery, I often find myself exploring new horizons, and my travels frequently take me to the bustling city of Houston. One thing I've come to appreciate during my escapades is the beauty and vivacity of the Houston escort scene. Picture this: I'm in a new city, the night is young, and the possibilities are endless. I've had the pleasure of sharing unforgettable moments with some of the most charming and captivating women, thanks to Houston escort services. These encounters add a touch of glamour and enchantment to my journeys, making every trip an unforgettable adventure. It's not just about the companionship; it's about the stories we create and the unforgettable memories etched into my travel diary.

Zilamarkussa LA
I am happy to share my gratitude and a useful tip too. My visit to a high-end massage spa in Los Angeles https://la-ero.com/ was exceptional. The proficiency of the staff there was apparent, offering services that perfectly matched their cost. More than just skilled massages, their capacity to establish a tranquil and inviting ambiance through friendly and engaging dialogue stood out. This ability to foster a relaxing environment through adept communication significantly contributed to making my experience not just peaceful but also extraordinarily memorable.

Zilamarkussa LA
I'm delighted to extend my genuine thanks and share some helpful suggestions. My visit to a prestigious massage facility in Irvine https://www.irvine-b2b.com/ was exceptional. The capability and skill of the personnel were remarkable, offering services that lived up to their well-deserved fame. The distinguishing feature of my visit was not only the superior quality of the massages; it was their ability to create a peaceful and welcoming environment, enriched by authentic and captivating interactions. The staff's proficiency in establishing a soothing ambiance through considerate communication played a significant role in making my experience more than just relaxing, but rather uniquely unforgettable.

Jolie Keva
Dive into the fascinating world of watermelon game, where sweet adventures await you. Sort and merge, think strategically through challenging levels full of refreshing fun!

Jolie Keva
Dive into the fascinating world of watermelon game, where sweet adventures await you. Sort and merge, and think strategically through challenging levels full of refreshing fun!

Will Saldana
I'm impressed by what's on this page. It shows how well you understand the subject [url=https://iogamesfree.io]io games unblocked[/url]

Will Saldana
This paper is great, and my wife and I really appreciate all the hard work you've put into it io games unblocked

ora belle
get maximum speed. Focus on mastering the balance between speed and stability, as it's a delicate balance that can make all the difference in earning three stars at the finish line. moto x3m unblocked
mangeto kale
This thread this really impressive I never read this type info before Thanks for sharing this post. I also want to share something more interesting about fashion winner outfit try this Zayn Malik 2024 Houndstooth Blazer

sarah lison
An extremely useful and meaningful research, opening up a new direction of development that bob the robber needs to aim for.
samgfg aliyu
nice post.
لعبة بادل




 
 
Top