Please login first
Light Sheet Fluorescence Microscopy using Incoherent Light Detection
* 1 , 2 , 1 , 1
1  University of Illinois at Chicago
2  Northern Arizona University
Academic Editor: VIJAYAKUMAR ANAND

Published: 13 March 2023 by MDPI in Holography Meets Advanced Manufacturing session Holography 2
Abstract:

We previously developed an incoherent holography technique for use in lattice light sheet (LLS) microscopes, that represents a specialized adaptation of light sheet microscopy. Light sheet instruments resolve 3D information by illuminating the sample at 90o to the imaging plane with a sheet of laser light that excites fluorophores in the sample only in a narrow plane. Imaging this plane and then moving it in the imaging z-axis allows construction of the sample volume. Among these types of instruments, LLS microscopy gives higher z-axis resolution and tissue depth penetration. It has a similar working principle to light sheet fluorescence microscopy, but uses a lattice configuration of Bessel beams, instead of Gaussian beams. Our incoherent light detection technique replaces the glass tube lens of the original LLS with a dual diffractive lens system to retrieve the axial depth of the sample. Here, we show that the system is applicable to the light sheet instruments. To make a direct comparison in the same emission light path, we can imitate the nature of non-Bessel light sheet systems by altering the mask annuli used to create Bessel beams in the LLS system. We change the diffractive mask annuli from a higher NA anulus to a smaller NA anulus. This generates a Gaussian excitation beam similar to conventional light sheet systems. Using this approach, we propose an incoherent light detection system for 3D imaging by choosing a variable NA and moving only the light sheet while keeping the sample stage and detection microscope objective stationary.

Keywords: Incoherent Holography; Light Sheet; Lattice Light Sheet; Fluorescence Microscopy; Diffractive Lenses
Comments on this paper
Adam Kris
Fluorescence microscopy can be used to: Observe the structure and properties of the sample: When the sample fluoresces, a fluorescence microscope allows you to observe the structure and properties of the sample in contrast, including both organic and inorganic samples. retro bowl
cuhn cuhnkedrik
The structure and qualities of a material can be studied with fluorescence microscopy by doing things like: Samples, both organic and inorganic, can be examined with a fluorescence microscope thanks to the contrast it provides between the sample's structure and qualities when it fluoresces. wordle today
baron meant
Enjoy a thrilling and action-packed drifting experience. Play drift boss game and practice your driving and drifting skills on winding tracks with lots of curves.

Andreaa Ferrason
The core technical premise is a perpendicularly positioned wide-field fluorescence microscope that gathers the fluorescence signal and photographs dino game of the viewed region using a full-frame camera.

norman helms
Your writing was entertaining to read. During the lean economic days, I used to kill time with a terrific game called pico park. Now follow my instructions.

Antonio Mays
The basic technical principle is a wide-field fluorescence microscope, placed perpendicular to the light-sheet, that collects the fluorescence signal and images of the observed region by means of a full-frame camera.

Slope Game
Single Shot Lensless Interferenceless Phase Imaging of Biochemical Samples Using Synchrotron near Infrared Beam. slopegameonline.io slope game

Tuan Cho
I have a wordle lot read many articles about microscope research, but your article is much more special. Through this article, I understand more about the structure of this type of glass. The article is very good and useful so I recommend you to publish more articles like this.
Dana Stherna
Well, if you want to tell if an aquarius man likes you or secretly has a crush on you, you need to observe his body language. Watch his legs, how he walks, looks at you and talks.

Tuan Cho
I have a wordle lot read many articles about microscope research, but your article is much more special. Through this article, I understand more about the structure of this type of glass. The article is very good and useful so I recommend you to publish more articles like this.

Tuan Cho
I have a [wordle](https://wordle-nyt.org/) lot read many articles about microscope research, but your article is much more special. Through this article, I understand more about the structure of this type of glass. The article is very good and useful so I recommend you to publish more articles like this.

Tuan Cho
I have a wordle lot read many articles about microscope research, but your article is much more special. Through this article, I understand more about the structure of this type of glass. The article is very good and useful so I recommend you to publish more articles like this.

Samuel Noble
Applying Light Sheet Fluorescence Microscopy using incoherent light detection has brought high efficiency in research. eggy car
Dana Stherna
Yes, sometimes, it really makes you wonder why are Gemini attracted to Capricorn since they are so different..almost like opposite zodiac signs. But my husband is Capricorn, I am a Gemini woman and we love each other and really get along well. So astrology can be accurate, in a way.

time line
Excellent material; I'll tell my friends about it and have them check it out. I really appreciate you sharing! When you have extra time, go to: run 3

Nhan Cho
With this wonderful guide from you, I was also able to know how to use a microscope to achieve the highest efficiency. This article is a sedecordle lot really useful for those interested in scientific topics.

problem trick
We appreciate you taking the time to provide us with this crucial information. You wrote a pretty well-written and insightful post.
Trickproblems

tella graph
Discover the latest tech trends with our in-depth articles, reviews, tips, tutorials, and news From AI to blockchain, virtual reality, cybersecurity, and more. Navigate the ever-changing landscape of tech with confidence. Checkout Tellagraph For More

tella graph
Discover the latest tech trends with our in-depth articles, reviews, tips, tutorials, and news From AI to blockchain, virtual reality, cybersecurity, and more. Navigate the ever-changing landscape of tech with confidence. Checkout Tellagraph For More

Vvansh Sharma
These premium girls are available at very cheap rate everyone can afford [url= https://www.girlsdelhi.in/saket-call-girls/] Saket Escorts Agency [/url] their budget it will not burden on your bank account. So don't hesitate to contact a Saket escorts so call us and book your girl and spent some unforgettable moments.

tella graph
The main purpose of a baby monitor is to watch your child when you’re not in the same room. There are several gadgets on the best non wifi baby monitor market that do far more than just fulfill their intended purpose.

loriwingler loriwingler
Онлайн казино БГ предлага на своите играчи https://онлайн-казино-бг.com/ невероятно разнообразие от азартни игри, които гарантират наистина вълнуващо преживяване. С богата селекция от слотове, покер, рулетка и други игри на късмет, тук всеки играч може да открие нещо, което му харесва. Уебсайтът предлага удобен и лесен за използване интерфейс, както и бързи и сигурни транзакции. Онлайн казино БГ съчетава забавлението с възможността за печалби, като създава идеална среда за игра и релаксация. Присъединете се сега и се потопете в света на азарта и развлечението!

tella graph
Genshin Leaks has revealed the five-star characters that will come in the next version, 4.8. Since the launch of the new Fontaine region, each update has added at least one new five-star character, with Clorinde being the most recent.

tella graph
Genshin Leaks has revealed the five-star characters that will come in the next version, 4.8. Since the launch of the new Fontaine region, each update has added at least one new five-star character, with Clorinde being the most recent.

Viet Heo
Thank you very much for giving me more information about Fluorescence Microscopy, I am researching this type of microscope and need a wordle nyt lot more information about it, luckily this article has provided some very useful information for me about this type of glasses.

Hazel H
Discover the latest tech trends with our in-depth articles, reviews, tips, tutorials, and news From AI to blockchain, virtual reality, cybersecurity, and more. getinstander.com/Instander apk Navigate the ever-changing landscape of tech with confidence.

Marvin Smith
A thin sheet of laser light is used to illuminate materials in LLS microscopy, a specialized type of light sheet microscopy that excites fluorophores exclusively in a predetermined plane mapsdirections.io/mapquest-directionsmapquest driving directions. This technique enables high-speed imaging while reducing photodamage.

gradecalculator grade
Wow! Thank you! I permanently needed to write on my website something like that When you are bored, visit this page, maybe it will help you solve the problems you are wondering about.final grade calculator




 
 
Top