Please login first
Light Sheet Fluorescence Microscopy using Incoherent Light Detection
* 1 , 2 , 1 , 1
1  University of Illinois at Chicago
2  Northern Arizona University
Academic Editor: VIJAYAKUMAR ANAND

Published: 13 March 2023 by MDPI in Holography Meets Advanced Manufacturing session Holography 2
Abstract:

We previously developed an incoherent holography technique for use in lattice light sheet (LLS) microscopes, that represents a specialized adaptation of light sheet microscopy. Light sheet instruments resolve 3D information by illuminating the sample at 90o to the imaging plane with a sheet of laser light that excites fluorophores in the sample only in a narrow plane. Imaging this plane and then moving it in the imaging z-axis allows construction of the sample volume. Among these types of instruments, LLS microscopy gives higher z-axis resolution and tissue depth penetration. It has a similar working principle to light sheet fluorescence microscopy, but uses a lattice configuration of Bessel beams, instead of Gaussian beams. Our incoherent light detection technique replaces the glass tube lens of the original LLS with a dual diffractive lens system to retrieve the axial depth of the sample. Here, we show that the system is applicable to the light sheet instruments. To make a direct comparison in the same emission light path, we can imitate the nature of non-Bessel light sheet systems by altering the mask annuli used to create Bessel beams in the LLS system. We change the diffractive mask annuli from a higher NA anulus to a smaller NA anulus. This generates a Gaussian excitation beam similar to conventional light sheet systems. Using this approach, we propose an incoherent light detection system for 3D imaging by choosing a variable NA and moving only the light sheet while keeping the sample stage and detection microscope objective stationary.

Keywords: Incoherent Holography; Light Sheet; Lattice Light Sheet; Fluorescence Microscopy; Diffractive Lenses
Comments on this paper
Adam Kris
Fluorescence microscopy can be used to: Observe the structure and properties of the sample: When the sample fluoresces, a fluorescence microscope allows you to observe the structure and properties of the sample in contrast, including both organic and inorganic samples. retro bowl
cuhn cuhnkedrik
The structure and qualities of a material can be studied with fluorescence microscopy by doing things like: Samples, both organic and inorganic, can be examined with a fluorescence microscope thanks to the contrast it provides between the sample's structure and qualities when it fluoresces. wordle today

Andreaa Ferrason
The core technical premise is a perpendicularly positioned wide-field fluorescence microscope that gathers the fluorescence signal and photographs dino game of the viewed region using a full-frame camera.

norman helms
Your writing was entertaining to read. During the lean economic days, I used to kill time with a terrific game called pico park. Now follow my instructions.

Antonio Mays
The basic technical principle is a wide-field fluorescence microscope, placed perpendicular to the light-sheet, that collects the fluorescence signal and images of the observed region by means of a full-frame camera.

Slope Game
Single Shot Lensless Interferenceless Phase Imaging of Biochemical Samples Using Synchrotron near Infrared Beam. slopegameonline.io slope game

Tuan Cho
I have a wordle lot read many articles about microscope research, but your article is much more special. Through this article, I understand more about the structure of this type of glass. The article is very good and useful so I recommend you to publish more articles like this.
Dana Stherna
Well, if you want to tell if an aquarius man likes you or secretly has a crush on you, you need to observe his body language. Watch his legs, how he walks, looks at you and talks.

Tuan Cho
I have a wordle lot read many articles about microscope research, but your article is much more special. Through this article, I understand more about the structure of this type of glass. The article is very good and useful so I recommend you to publish more articles like this.

Tuan Cho
I have a [wordle](https://wordle-nyt.org/) lot read many articles about microscope research, but your article is much more special. Through this article, I understand more about the structure of this type of glass. The article is very good and useful so I recommend you to publish more articles like this.

Tuan Cho
I have a wordle lot read many articles about microscope research, but your article is much more special. Through this article, I understand more about the structure of this type of glass. The article is very good and useful so I recommend you to publish more articles like this.

Samuel Noble
Applying Light Sheet Fluorescence Microscopy using incoherent light detection has brought high efficiency in research. eggy car
Dana Stherna
Yes, sometimes, it really makes you wonder why are Gemini attracted to Capricorn since they are so different..almost like opposite zodiac signs. But my husband is Capricorn, I am a Gemini woman and we love each other and really get along well. So astrology can be accurate, in a way.

time line
Excellent material; I'll tell my friends about it and have them check it out. I really appreciate you sharing! When you have extra time, go to: run 3

Nhan Cho
With this wonderful guide from you, I was also able to know how to use a microscope to achieve the highest efficiency. This article is a sedecordle lot really useful for those interested in scientific topics.

problem trick
We appreciate you taking the time to provide us with this crucial information. You wrote a pretty well-written and insightful post.
Trickproblems

tella graph
Discover the latest tech trends with our in-depth articles, reviews, tips, tutorials, and news From AI to blockchain, virtual reality, cybersecurity, and more. Navigate the ever-changing landscape of tech with confidence. Checkout Tellagraph For More

tella graph
Discover the latest tech trends with our in-depth articles, reviews, tips, tutorials, and news From AI to blockchain, virtual reality, cybersecurity, and more. Navigate the ever-changing landscape of tech with confidence. Checkout Tellagraph For More




 
 
Top