Bandages, Gauzes and Alcoholic pads have widely been used for wound healing. However due to few limitations hydrophilic membranes loaded with nanoparticles have been explored rarely as a wound healing material. This study aimed to synthesize and characterize Polyvinyl Alcohol (PVA) and Gum Tragacanth(GT) membrane having different concentrations of gold nanoparticles(AuNPs) loaded with Ciprofloxacin as bacterial infection is one of the major problem for wound healing. Gold and CIP-loaded nanoparticles were successfully prepared by Turkevich method and confirmed by Fourier-transform infrared spectroscopy. To analyze the hydrophilicity of membrane swelling test as well as contact angle analysis were performed. In summary, this study demonstrated that hydrophilic membrane of PVA/GUM T. having 500ul concentration of CIP-loaded AuNPs can be used as wound healing materials without affecting their biocompatibility.