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Prevalence of antibiotic resistance of uropathogenic bacteria isolated from contaminated urine
* 1 , 2, 3 , 1 , 1
1  Department of Living and Environment, Faculty of Natural Sciences and Life, University of Science and Technology Mohamed BOUDIAF, USTOMB, P.B. 1505, El Mnaouar, Bir El Djir 31000, Oran, Algeria.
2  Higher School of Biological Sciences of Oran
3  Laboratory of Micro-organisms Biology and Biotechnology, University of Oran 1, P.B. 1524, El M’Naouer, 31000 Oran, Algeria.
Academic Editor: Marc Maresca

Published: 30 November 2023 by MDPI in The 3rd International Electronic Conference on Antibiotics session Poster Session

The prevalence of resistance to antibiotics of uropathogenic bacteria constitute a very major health problem and it is the subject of much research. The inhibition of the uropathogenic bacteria by lactic acid bacteria is the subject of a number of studies. In this study, we evaluate the resistance, the multi-resistance and the susceptibility of some bacteria isolated from contaminated urine and their inhibition by three lactic acid bacteria isolated from feed: Enterococcus faecium CM9, Enterococcus faecium H3 and Lactobacillus brevis LBM2.

Methods. The evaluation of the resistance to antibiotics of uropathogenic bacteria was evaluated by the Vitek 2 Compact using an adequate card and were performed as the standard procedure. The inhibition of the uropathogenic bacteria by the lactic acid bacteria strains was performed using the streak agar test described by Ayeni et al. (2009).

Results. Ten uropathogenic strains from urine samples from patients with urinary tract infections were isolated, it's about: Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Serratia marcescens, Staphylococcus aureus, Staphylococcus saprophyticus, Sterptococcus agalactiae, Entrobacter cloacae. The antibiogram test expressed by Vitek 2 Compact revealed that Klebsiella pneumoniae was the most resistant to antibiotics, while Escherichia coli was the most sensitive. The study also showed that three lactic strains Lactobacillus brevis LBM2, Enterococcus faecium CHM9 and H2.3 had strong antimicrobial activity against Gram-positive and Gram-negative uropathogen bacteria.

Keywords: uropathogen bacteria ; antibiotic resistance ; ECBU ; Vitek 2 Compact ; lactic acid bacteria ; inhibitory activity.