A comparative study of the ethanol extract of the leaves of four species of Psidium (P. cattleianum, P. claraense, P. guajava and P. sartorianum) was carried out. The investigation included phytochemical screening, Folin-Ciocalteu method to determine total phenolic content and determination of antioxidant activity by assaying the free radical sequestering activity of 2,2-diphenyl-1-picryl-hydrazyl (DPPH). The phytochemical screening showed a similar composition in the four evaluated species. The phenolic content of the ethanolic extracts of the four species was high, especially in the extract of Psidium sartorianum (17.59 mgEAG / gES) but lower than that obtained for Psidium guajava (19.782 mgEAG / gES) considered a reference species. The ethanolic extracts of the four species of Psidium showed values ​​of DPPH free radical scavenger activity higher than the synthetic antioxidant Butyl hydroxytoluene (BHT) (175.98 μg / mL), highlighting the Psidium sartorianum (17.82 μg / mL).