Background: Chasmanthera dependens is a medicinal plant with wide application in African traditional medicine for the management of several pathologies. The phytochemistry, antiulcerogenic properties and antimicrobial properties have been reported and attributed to its robust phytochemistry. We hereby report the antioxidant properties of the chloroform extract of C. Dependens root as to provide further scientific information for the explanation of some of the reported properties of the plant in human diseases. Methods: In vitro studies assayed for diphpnylpicryl hydrazyl (DPPH) scavenging, ferric reducing antioxidant power (FRAP), hydrogen peroxide scavenging and total antioxidant capacity (TAC). Invivo studies assayed for superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) on CCl₄-induced wistar rats. Standard methods were followed in all assays. Results: DPPH radical scavenging activity was concentration dependent with an EC₅₀ of 647.67μg/ml. The extract showed a positive hydrogen peroxide scavenging with EC₅₀ of 57.78±2.93μg/ml relative to ascorbic acid standard (EC₅₀: 90.06μg/ml). The ferric reducing antioxidant power (FRAP) at the concentration 15.6μg/ml was 21.18±0.15 gallic acid equivalent while that of 1000μg/ml was 0.03±0.00 gallic acid equivalent for the extract. Total antioxidant capacity (TAC) at a concentration 15.6μg/ml showed a total antioxidant capacity of 15.22±7.81 ascorbic acid equivalent while that of 1000 μg/ml was 0.03±0.00 for the extract. Result of in vivo antioxidant studies showed a significant increase (p < 0.05) in the concentrations of endogenous antioxidant enzymes in the treated groups relative to the untreated control. Conclusion: we therefore conclude that the observed antioxidant properties of CECDR could be attributed to its wealth of phytochemical constituents as reported by previous studies. This study also highlights the possible application of CECDR as part of drug discovery raw material.