Introduction: The prostate is an exocrine gland regulated by testosterone. This gland is target of proliferative lesions, such as benign prostatic hyperplasia and cancer, which is related to the testosterone synthesis augment. The search for anticancer natural substances capable of preventing/treating proliferative lesions has fostered interest in therapeutic properties of Pequi (Caryocar brasiliense), known to be composed of various antioxidant, anti-inflammatory and anti-proliferative substances. Thus, the objective of this study was to verify the anti-proliferative property of pequi oil in the gerbil prostate under testosterone influence. Methodology: The animals were divided into the following groups: T (subcutaneous administration of testosterone cypionate; T+P (pequi oil and testosterone cypionate) and Control (intact animals). After animal death, the prostates were collected and embedded in paraffin for Haematoxylin-Eosin stain, for histopathological evaluation, and anti-PCNA immunostain for proliferative activity analysis. Results: Morphological analysis showed presence of stratification in the prostate epithelium of group T animals, accompanied by a high number of PCNA + cells, showing a high proliferative activity. In the T + P group, the epithelium presented a pseudostratification pattern, with a smaller number of PCNA + cells, similar to the Control group, showing a decrease in proliferative activity and maintenance of the normal prostate tissue architecture when compared to the T group. Conclusion: Our results show an anti-proliferative effect of pequi oil on the gerbil prostate under the influence of exogenous testosterone. Thus, pequi oil has great potential in the prevention of prostate proliferative lesions, such as cancer.

Guarana or Paullinia cupana var. Sorbilis (Mart.) Ducke, since it has relevant therapeutic effects, has been the subject of nanotechnology study in order to magnify its activity. In the article Protective effect of liposomes loaded with guarana on the hemolytic activity of author I Roggia, a study was conducted where physicochemical parameters were evaluated to determine the stability of liposomes containing guarana. To evaluate the hemolytic and antioxidant activity, the red blood cells of the rats were subjected to different formulations dissolved in PBS (Guarana liposomal, free form and liposomal guarana without guarana). These formulations were compared and the most stable results were those of one containing 1mgml-1 of guarana by the reverse phase evaporation method. On the other hand, the formulation of guarana in free form has dose-dependent antioxidant properties and the liposomal formulation without guarana hemolytic activity before polysorbate 80 present in the PBS of the buffer solution.

For the study of enzymatic designs and directed evolution projects, a relevant program would be Transform-MinER. In the article Exploring the space of the biosynthetic chemical design with Transorm-MinER, it is explained that this by means of molecular transformations in enzymatic reactions, allows to explore the chemical space by searching for a starting point. With the use of chemoinformatic fingerprints, it searches for possible reaction centers in the source substrate and identifies those in environments similar to those of the natives. It uses two types of search for enzymatic reactions in similar substrates and for enzymatic reactions that link the source and the target with the sending of a target molecule (molecule search and route search, respectively). Through the KEEG database, which is nothing more than a collection of database of genomes and metabolic pathways and de novo enzymatic reaction data, its performance is determined, with a 90% success considered optimal. On the other hand, the article Transform - MinER: transforming molecules into enzymatic reactions, reaffirms the fact that said program facilitates the identification of initial enzymes in an investigation, since it finds the most similar native enzymatic reactions emphasizing those of greater interest to the researcher .

Diphtheria is an infectious disease prevented by vaccination. To ensure the

safety and immunogenicity of these vaccines, the regulatory entities demand in

vitro and in vivo techniques. In vitro techniques, such as ELISA, reduce the

number of animals, are more sensitive, faster and cheaper, then, currently work

is focused on its implementation. Therefore, the purpose of this work was to

develop a serological assay, ELISA in curiel, that allow the determination of the

immunogenic activity of this type of vaccines. The batches of reference

materials (RM) were prepared: Diphtheria coating toxoid, curiel serum,

calibration curve and positive control. The optimal conditions of the assay were

established, the interval and linearity of the curve were defined, as well as the

intra- and inter-assay precision and the specificity. The results of the serological

test, ELISA in curiel, and the in vivo seroneutralization test dose were

compared. The calibration curve was generated with a standard serum and a

good linear adjustment was achieved with a R2 ≥ 0,98; stablishing 10Lf/mL as

the optimal coating concentration, 1/12000 as the dilution of conjugate and an

incubation temperature of 37ºC. The variation coefficients in the intra- and inter

assay precision tests in the intervals established for each one were ≤ 10% and

≤ 20% respectively, showing an adequate correlation between both tests. The

results obtained endorse the employment of this quantitative ELISA for the

evaluation of the immunogenic activity of antidiphtheric vaccines.

Temperature programming is one of the techniques to achieve selectivity with metal oxide semiconductor (MOS) gas sensors. Here, we report a study on the response of ZnO nanostructures based H₂S sensor at different temperatures. It was observed that the response didn’t exhibit the same behavior at all concentrations with increase in temperature. It can be attributed to adsorbed oxygen molecules, adsorption-desorption rate and number of surface charge carriers, which vary with temperature. This variation in sensor response with temperature for different concentration can be useful in developing single sensor based electronic nose to detect multiple gases selectively.

The objective of this article is the literature review of the components of the study of Tardigrada or (water bear), followed by the analysis of the information to describe the main peculiarities of this organism, with the objective of determining the importance of the water bear to knowledge of new technological applications (such as a protein from Tardigrada protects human DNA from sunlight) and many other applications offered by this organism, which due to lack of information limits understanding as a fundamental source for future applicable research in the study of this organism. With a description of their cryptobiosis states. The research will also contain a sample of the Tardigrada species in the tourist hike of the Puyo River (Pastaza province), increasing the knowledge of its distribution, in which the abundance existing in the area will be measured. A sample of the tardigrade will be taken in three different parts of the place, with strata of tree bark and stone to continue observing them in a digital microscope, followed by the identification of the class with its respective taxonomic key.

Influence of discharge from the State Amazon University (UEA) on the ichthyological diversity of the river Puyo, since Guevara & Ramos in 2018 stated that there is a 39% efficiency of the Wastewater Treatment Plant (PTAR) therefore several fish species are relegated from their habitat, for the determination of the study area the capture method was used by delimiting the discharge point of waste water as the starting area and the catches 1000 metres upwards, as well as 1000 metres downwards, by marking four sampling points in the above-mentioned areas, 30 minutes per sampling area were used using an atarraya to capture and photograph species within a 30-metre radius at the centre of the sampling points, 24 specimens from six families (Anostomidae, Cichlidae, Curimatidae, Characidae, Heptapteridae &Loricariidae), belonging to three orders (Characiformes, Perciformes & Siluriformes) where the predominant family is Loricariidae with 17 individuals, in the lower part is recorded a higher rate of orders versus the upper part in which two orders of the three obtained in total of the study are recorded.

The objective of the research was to capture and identify the diversity of invertebrates that comprise an intervened area and that of a secondary forest. Classifying them into classes and orders, to which each one of the individuals obtained in the transects belongs, comparing each specific place and analyzing the variety that each zone presents. The aggressive manifestation of anthropogenic factors has modified the existing balance in the environment, which is affecting the biodiversity of the living beings that inhabit it. One of the aspects taken into account in this research, is the most diverse group of living beings invertebrates, so we have seen the need to study the degree of disturbance in two particular sites, based on the latent diversity of each territory.

Identificar el rol que presenta cada una de las especies que forma la microbiota es de vital importancia para el futuro tratamiento de las enfermedades. El Dr. Cesar de la Fuente nos muestra una manera de diseñar péptidos que puedan matar a especies bacterianas de forma específica, con el fin de crear modelos de animales ‘knock out’ microbióticos que se puedan estudiar en el laboratorio. La investigación la realiza a partir de técnicas computacionales como los algoritmos genéticos, que siguiendo las bases de la evolución de Darwin, son capaces de diseñar teóricamente péptidos cada vez más efectivos contra ciertas bacterias.

Link al vídeo: https://www.youtube.com/watch?v=aPuUn-iJF9s&t=310s

Introducción a I International Conference and Workshop: Microbiota: What’s
cooking? por el Dr. Alejandro Pazos Sierra.
Se entiende por microbiota al conjunto de microorganismos que se en-
cuentran en distintas partes del cuerpo humano. Los microorganismos resi-
den en estos lugares de forma más o menos permanente y en algunos casos
realizan funciones especı́ficas, pudiendo ser la causa de muy diferentes pa-
tologı́as, tanto a nivel local como a distancia. Estos microorganismos son
predominantemente bacterias, aunque también se encuentran virus, hon-
gos, arqueas, etc. Se entiende que nos acompañan a cada uno de nosotros
aproximadamente 10 14 de estos microorganismos.
Microbioma se considera al conjunto de genes de los citados microorga-
nismos presentes en nuestra economı́a. Este material genético puede inter-
actuar, localmente o a distancia, con proteı́nas de células diana pudiendo
causar disfunciones que den lugar a distintas patologı́as.

Link al vı́deo: https://www.youtube.com/watch?v=Oy3openHPN8