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Single Shot Lensless Interferenceless Phase Imaging of Biochemical Samples Using Synchrotron Near Infrared Beam
1 , 1 , 1 , 1 , 2 , 2 , 3 , 3 , 3 , 1, 4 , * 1, 2
1  Optical Sciences Centre and ARC Training Centre in Surface Engineering for Advanced Materials (SEAM), School of Science, Swinburne University of Technology, Hawthorn, VIC 3122, Australia
2  Institute of Physics, University of Tartu, W. Ostwaldi 1, 50411 Tartu, Estonia
3  Infrared Microspectroscopy (IRM) Beamline, ANSTO—Australian Synchrotron, Clayton, VIC 3168
4  Tokyo Tech World Research Hub Initiative, School of Materials and Chemical Technology, Tokyo Institute of Technology, 2-12-1, Ookayama, Meguro-ku, Tokyo 152-8550, Japan
Academic Editor: VIJAYAKUMAR ANAND

Published: 13 March 2023 by MDPI in Holography Meets Advanced Manufacturing session Holography 1
https://doi.org/10.3390/HMAM2-14150 (registering DOI)
Abstract:

Near-IR (NIR) region of the synchrotron-IR beam, which is usually filtered out in order to improve the signal to noise ratio of Fourier-transform infrared spectroscopy (FTIR), was extracted and used as an illuminating source to achieve phase imaging of biochemical samples. A 200-um pinhole was aligned with one lobe of the unique fork shaped NIR synchrotron beam at a resulting intensity maximum. The diffracted light with airy diffraction pattern passed the biochemical sample and was collected by NIR sensitive lensless camera. The Gerchberg-Saxton algorithm (GSA) was used to reconstruct the phase images of the samples from recorded the intensity images.

Keywords: phase imaging, bioimaging, synchrotron, near infrared beam, holography, incoherent optics, chemical imaging, phase retrieval, 3D imaging
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