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Single Shot Lensless Interferenceless Phase Imaging of Biochemical Samples Using Synchrotron Near Infrared Beam
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Mark J Tobin
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Academic Editor: VIJAYAKUMAR ANAND
https://doi.org/10.3390/HMAM2-14150
(registering DOI)
Abstract:
Near-IR (NIR) region of the synchrotron-IR beam, which is usually filtered out in order to improve the signal to noise ratio of Fourier-transform infrared spectroscopy (FTIR), was extracted and used as an illuminating source to achieve phase imaging of biochemical samples. A 200-um pinhole was aligned with one lobe of the unique fork shaped NIR synchrotron beam at a resulting intensity maximum. The diffracted light with airy diffraction pattern passed the biochemical sample and was collected by NIR sensitive lensless camera. The Gerchberg-Saxton algorithm (GSA) was used to reconstruct the phase images of the samples from recorded the intensity images.
Keywords: phase imaging, bioimaging, synchrotron, near infrared beam, holography, incoherent optics, chemical imaging, phase retrieval, 3D imaging
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