Calcium carbonate nanoparticles have salient properties, such as biocompatibility, pH responsiveness, and the ability to alkalinize a tumor, hence reducing metastasis. A combination therapy regimen is normative for breast cancer, where, besides its side effects, toxic vehicles are required for certain drugs. This study is aimed at transforming the readily available Blood cockle shells (Anadara granosa) to calcium carbonate nanoparticles (CSCaCO3NP), loading them with Gefitinib (GEF) and Paclitaxel (PTXL). Facile top-down synthesis of CSCaCO3NP is comprised of grinding, sieving, and stirring with Tween 80, followed by filtration and finally dry milling for 100 hours. A ratio of 1+0.5:25 of GEF+PTXL: CSCaCO3NP in an equal admixture of DMSO and 0.05%Tween 80 buffer was used for drug loading. Loading content (%) and encapsulation efficiency (%) for GEF and PTXL in dual drug-loaded NP (GEF-PTXL-CSCaCO3NP) was 1.98 ± 0.11, 50.01 ± 2.18 and 0.92 ± 0.01, 45.60 ± 0.32. Field emission Scanning electron micrographs revealed that the nanoparticles were almost spherical with the average diameter (nm) measuring 63.96 ± 22.3 and 87.20 ± 26.66 for CSCaCO3NP and GEF-PTXL-CSCaCO3NP. The Dynamic Light Scattering data gives the average diameter of CSCaCO3NP and GEF-PTXL-CSCaCO3NP as 179 ± 10.9 (nm) and 274 ± 23.22 (nm), respectively, and polydispersive index of 0.3. Zeta potential was -17 ± 1.15 (mV) and 10.30 ± 1.7 (mV), respectively. Fourier-transform Infrared spectroscopy proves that CSCaCO3NP has encapsulated the drugs. X-Ray Diffraction data indicates that the aragonite phase is unaltered. N2 adsorption-desorption isotherms reveal that CSCaCO3NP are mesoporous and that the surface area had reduced from 10.68 ± 0.22 to 9.88 ± 0.24 m2/g after drug loading. For the first time, this work will describe the process to synthesize CSCaCO3NP which was used as a carrier to load GEF and PTXL and its salient characteristics.
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Synthesis and characterization of Gefitinib and Paclitaxel dual drug loaded Cockle shell (Anadara granosa) derived Calcium carbonate nanoparticles
Published:
10 November 2020
by MDPI
in 2nd International Online-Conference on Nanomaterials
session Synthesis and Characterization of Nanomaterials
Abstract:
Keywords: Calcium carbonate nanoparticles; Cockle shells; Gefitinib; Paclitaxel; Dual drug loading; FESEM; XRD; FTIR;
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