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Franck Vazquez   Dr.  President, CEO or Director 
Affiliations
MDPI, Basel, Switzerland
Timeline See timeline
Franck Vazquez published an article in November 2014.
Research Keywords & Expertise
0 Plant Biology
1 RNA
0 RNAi
0 SiRNA
0 Virology
0 microRNA
Top co-authors See all
George A. Calin

263 shared publications

Department of Experimental Therapeutics, The University of Texas MD Anderson Cancer Center, Houston, 1515 Holcombe Boulevard, Unit 422, Houston, TX 77030, USA

Rob W. Briddon

202 shared publications

Agricultural Biotechnology Division, National Institute for Biotechnology and Genetic Engineering, Faisalabad 38000, Pakistan

Hervé Vaucheret

135 shared publications

Institut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, Université Paris-Saclay, 78026 Versailles Cedex, France

Gaurav Sablok

50 shared publications

Finnish Museum of Natural History, Helsinki, Finland

Tatiana V. Tatarinova

50 shared publications

Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, Russia

11
Publications
9
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Downloads
187
Citations
Publication Record
Distribution of Articles published per year 
(2008 - 2014)
Total number of journals
published in
 
9
 
Publications See all
Article 1 Read 0 Citations Welcome to the New Journal Non-Coding RNA! George A. Calin, Franck Vazquez Published: 21 November 2014
Non-Coding RNA, doi: 10.3390/ncrna1010001
DOI See at publisher website ABS Show/hide abstract
Note: In lieu of an abstract, this is an excerpt from the first page.Excerpt Dear colleagues and non-coding RNAs aficionados, you will probably ask 'why a new journal'?, these days when we are flooded not only by information of any type in any sector of our life, but also by so many new journals that come and disappear like comets in the summer sky! The answer is simple: because we believe that this field finally deserves to have a dedicated journal where its wide community will be able to communicate and exchange its latest findings in one centralized place. Moreover, with your outstanding contributions and by publishing papers that promulgate the 'thinking out of the box', we will be able to build a reputation for Non-Coding RNA to survive over the years to come.
Article 2 Reads 10 Citations The crosstalk between plant microRNA biogenesis factors and the spliceosome Zofia Szweykowska-Kulinska, Artur Jarmolowski, Franck Vazque... Published: 03 December 2013
Plant Signaling & Behavior, doi: 10.4161/psb.26955
DOI See at publisher website PubMed View at PubMed ABS Show/hide abstract
Many of the plant microRNA (miRNA) genes contain introns. The miRNA-containing hairpin loop structure is predominantly located within the first exon of such pri-miRNAs. We have shown that the downstream intron and its splicing are important for the regulation of the processing of these pri-miRNAs. The 5′ splice site in MIR genes is essential in the process of miRNA biogenesis. We postulate that the presence of yet undefined interactions between U1 snRNP and the pri-miRNA processing machinery leads to an increase in the efficiency of miRNA biogenesis. The 5′ splice site also decreases the accessibility of the polyadenylation machinery to the pri-miRNA polyA signal located within the same intron. It is likely that the spliceosome assembly controls the length and structure of MIR primary transcripts, and regulates mature miRNA levels. The emerging picture shows that introns, splicing, and/or alternative splicing have highly relevant roles in regulating the miRNA levels in very specific conditions that contribute to proper plant response to stress conditions.
Article 1 Read 32 Citations Introns of plant pri-miRNAs enhance miRNA biogenesis Dawid Bielewicz, Malgorzata Kalak, Maria Kalyna, David Winde... Published: 17 May 2013
EMBO Reports, doi: 10.1038/embor.2013.62
DOI See at publisher website PubMed View at PubMed ABS Show/hide abstract
MiRNA-proximal introns and functional splice sites are required for the accumulation of normal levels and the activity of mature miRNAs in Arabidopsis.
Article 0 Reads 11 Citations Biogenesis and Biological Activity of Secondary siRNAs in Plants Franck Vazquez, Thomas Hohn Published: 12 February 2013
Scientifica, doi: 10.1155/2013/783253
DOI See at publisher website PubMed View at PubMed ABS Show/hide abstract
Two important hallmarks of RNA silencing in plants are (1) its ability to self-amplify by using a mechanism called transitivity and (2) its ability to spread locally and systemically through the entire plant. Crucial advances have been made in recent years in understanding the molecular mechanisms of these phenomena. We review here these recent findings, and we highlight the recently identified endogenous small RNAs that use these advantageous properties to act either as patterning signals in important developmental programs or as a part of regulatory cascades.
Article 0 Reads 5 Citations Transcript mapping of Cotton leaf curl Burewala virus and its cognate betasatellite, Cotton leaf curl Multan betasatelli... Fazal Akbar, Rob W Briddon, Franck Vazquez, Muhammad Saeed Published: 29 October 2012
Virology Journal, doi: 10.1186/1743-422X-9-249
DOI See at publisher website PubMed View at PubMed ABS Show/hide abstract
Whitefly-transmitted geminiviruses (family Geminiviridae, genus Begomovirus) are major limiting factors for the production of numerous dicotyledonous crops throughout the warmer regions of the world. In the Old World a small number of begomoviruses have genomes consisting of two components whereas the majority have single-component genomes. Most of the monopartite begomoviruses associate with satellite DNA molecules, the most important of which are the betasatellites. Cotton leaf curl disease (CLCuD) is one of the major problems for cotton production on the Indian sub-continent. Across Pakistan, CLCuD is currently associated with a single begomovirus (Cotton leaf curl Burewala virus [CLCuBuV]) and the cotton-specific betasatellite Cotton leaf curl Multan betasatellite (CLCuMuB), both of which have recombinant origins. Surprisingly, CLCuBuV lacks C2, one of the genes present in all previously characterized begomoviruses. Virus-specific transcripts have only been mapped for few begomoviruses, including one monopartite begomovirus that does not associate with betasatellites. Similarly, the transcripts of only two betasatellites have been mapped so far. The study described has investigated whether the recombination/mutation events involved in the evolution of CLCuBuV and its associated CLCuMuB have affected their transcription strategies. The major transcripts of CLCuBuV and its associated betasatellite (CLCuMuB) from infected Nicotiana benthamiana plants have been determined. Two complementary-sense transcripts of ~1.7 and ~0.7 kb were identified for CLCuBuV. The ~1.7 kb transcript appears similar in position and size to that of several begomoviruses and likely directs the translation of C1 and C4 proteins. Both complementary-sense transcripts can potentially direct the translation of C2 and C3 proteins. A single virion-sense transcript of ~1 kb, suitable for translation of the V1 and V2 genes was identified. A predominant complementary-sense transcript was also confirmed for the betasatellite. Overall, the transcription of CLCuBuV and the recombinant CLCuMuB is equivalent to earlier mapped begomoviruses/betasatellites. The recombination events that featured in the origins of these components had no detectable effects on transcription. The transcripts spanning the mutated C2 gene showed no evidence for involvement of splicing in restoring the ability to express intact C2 protein.
Article 0 Reads 39 Citations Primary and Secondary siRNAs in Geminivirus-induced Gene Silencing Michael Aregger, Basanta K. Borah, Jonathan Seguin, Rajendra... Published: 27 September 2012
PLOS Pathogens, doi: 10.1371/journal.ppat.1002941
DOI See at publisher website PubMed View at PubMed ABS Show/hide abstract
In plants, RNA silencing-based antiviral defense is mediated by Dicer-like (DCL) proteins producing short interfering (si)RNAs. In Arabidopsis infected with the bipartite circular DNA geminivirus Cabbage leaf curl virus (CaLCuV), four distinct DCLs produce 21, 22 and 24 nt viral siRNAs. Using deep sequencing and blot hybridization, we found that viral siRNAs of each size-class densely cover the entire viral genome sequences in both polarities, but highly abundant siRNAs correspond primarily to the leftward and rightward transcription units. Double-stranded RNA precursors of viral siRNAs can potentially be generated by host RDR-dependent RNA polymerase (RDR). However, genetic evidence revealed that CaLCuV siRNA biogenesis does not require RDR1, RDR2, or RDR6. By contrast, CaLCuV derivatives engineered to target 30 nt sequences of a GFP transgene by primary viral siRNAs trigger RDR6-dependent production of secondary siRNAs. Viral siRNAs targeting upstream of the GFP stop codon induce secondary siRNAs almost exclusively from sequences downstream of the target site. Conversely, viral siRNAs targeting the GFP 3′-untranslated region (UTR) induce secondary siRNAs mostly upstream of the target site. RDR6-dependent siRNA production is not necessary for robust GFP silencing, except when viral siRNAs targeted GFP 5′-UTR. Furthermore, viral siRNAs targeting the transgene enhancer region cause GFP silencing without secondary siRNA production. We conclude that the majority of viral siRNAs accumulating during geminiviral infection are RDR1/2/6-independent primary siRNAs. Double-stranded RNA precursors of these siRNAs are likely generated by bidirectional readthrough transcription of circular viral DNA by RNA polymerase II. Unlike transgenic mRNA, geminiviral mRNAs appear to be poor templates for RDR-dependent production of secondary siRNAs.
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