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Aldo G.M. Brinkman  - - - 
Top co-authors
Ernst J. R. Sudhölter

45 shared publications

Department of Chemical Engineering, Delft University of Technology, Van der Maasweg 9, 2629 HZ Delft, The Netherlands

Johan H. Klootwijk

12 shared publications

C Philips Research, High Tech Campus 4, 5656 AE, Eindhoven, The Netherlands

Marleen Mescher

7 shared publications

Chemical Engineering, Delft University of Technology, 2628 BL Delft, The Netherlands.

Duco Bosma

6 shared publications

Department of Chemical Engineering, Delft University of Technology, Van der Maasweg 9, 2629 HZ Delft, The Netherlands

Publication Record
Distribution of Articles published per year 

Total number of journals
published in
Article 0 Reads 3 Citations A generic microfluidic biosensor of G protein-coupled receptor activation – impedance measurements of reversible morphol... Saurabh K. Srivastava, Rajesh Ramaneti, Margriet Roelse, Hie... Published: 01 January 2015
RSC Advances, doi: 10.1039/C5RA04976H
DOI See at publisher website ABS Show/hide abstract
Flowcell with micro-IDEs (250–500 μm) covered with both stable and reverse transfected cells overexpressing membrane receptors to demonstrate impedance responses to serial injections of analyte. Impedance spectroscopy of cell lines on interdigitated electrodes (IDEs) is an established method of monitoring receptor-specific cell shape changes in response to certain analytes. Normally, assays are done in multiwells making it a bulky, static and single use procedure. Here, we present a biosensor allowing sequential application of biological test samples with an automated microfluidic system. It is capable of monitoring relative changes in impedance using castellated IDEs of 250–500 μm diameter, covered with stable or reverse transfected HEK293 cells. Reversible activation of the Neurokinin 1 (NK1) receptor in stable cell lines was observed in response to a series of 5 minute exposures from 1 pM–10 nM of the specific ligand Substance P (SP) using impedance measurements at 10 mV and 15 kHz. An optimal flow speed of 10 μl min −1 was chosen for the 10 μl flow cell. The EC 50 of ∼10 pM was about 10 times lower than the EC 50 based on measuring changes in the calcium ion concentration. The method was also shown to work with reverse transfected cells. Plasmid DNA encoding the NK1 gene was spotted onto the electrodes and pre-incubated with a transfection agent. The overlaid HEK293 cells were subsequently transfected by the underlying DNA. After challenge with SP, the cells induced an activation response similar to the stable cell line. The microfluidic micro-electrode reverse transfection system opens up possibilities to perform parallel measurements on IDE arrays with distinct receptors per IDE in a single flow channel.
Article 3 Reads 3 Citations Influence of conductivity and dielectric constant of water-dioxane mixtures on the electrical response of SiNW-based FET... Marleen Mescher, Aldo G.M. Brinkman, Duco Bosma, Johan H. Kl... Published: 29 January 2014
Sensors, doi: 10.3390/s140202350
DOI See at publisher website PubMed View at PubMed ABS Show/hide abstract
In this study, we report on the electrical response of top-down, p-type silicon nanowire field-effect transistors exposed to water and mixtures of water and dioxane. First, the capacitive coupling of the back gate and the liquid gate via an Ag/AgCl electrode were compared in water. It was found that for liquid gating smaller potentials are needed to obtain similar responses of the nanowire compared to back gating. In the case of back gating, the applied potential couples through the buried oxide layer, indicating that the associated capacitance dominates all other capacitances involved during this mode of operation. Next, the devices were exposed to mixtures of water and dioxane to study the effect of these mixtures on the device characteristics, including the threshold voltage (V(T)). The V(T) dependency on the mixture composition was found to be related to the decreased dissociation of the surface silanol groups and the conductivity of the mixture used. This latter was confirmed by experiments with constant conductivity and varying water-dioxane mixtures.