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Eric Gontier  - - - 
Publication Record
Distribution of Articles published per year 
(1991 - 2018)
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Article 0 Reads 0 Citations Tropane alkaloids GC/MS analysis and low dose elicitors’ effects on hyoscyamine biosynthetic pathway in hairy roots of A... Boualem Harfi, Lakhdar Khelifi, Majda Khelifi-Slaoui, Corinn... Published: 18 December 2018
Scientific Reports, doi: 10.1038/s41598-018-36625-4
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Plant secondary metabolites play a major role in plant adaptation to stress. Species belonging to Solanaceae family and Datura genus produce tropane alkaloids: D. stramonium, D. tatula and D. innoxia. These species are cultivated for their hyoscyamine (HS) content, whence the interest of this study to induce transformed roots of these species with strain A4 of Agrobacterium rhizogenes. Hairy roots (HRs) of Datura were established at high frequency by infecting vitroplants. All HRs (343 independent lines) were next employed to study the production of HS and growth. A screening of HRs alkaloid content by GC/MS is performed; it reveals, for the first time, the production of 13 alkaloids by the selected root lines. The selection of high productive line offers an interesting option to enhance the productivity. As HS is the dominant compound, the lines of Datura species were selected for their characteristics for biomass and HS production. The elicitors salicylic acid (SA) and acetyl salicylic acid (ASA) were also used to increase HS production. The results showed that the optimal concentration of the two elicitors (AS and ASA) was 0.1 mM. The highest HS content (17.94 ± 0.14 mg g−1 D.W.) obtained in HRs of D. tatula treated with 0.1 mM of acetyl salicylic acid.
Article 0 Reads 0 Citations Datura innoxia plants hydroponically-inoculated with Agrobacterium rhizogenes display an enhanced growth and alkaloid me... Thi Dao Vu, Cyril Jousse, Nathalie Pawlicki-Jullian, Séverin... Published: 01 December 2018
Plant Science, doi: 10.1016/j.plantsci.2018.09.001
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Article 0 Reads 0 Citations The Desirability Optimization Methodology; a Tool to Predict Two Antagonist Responses in Biotechnological Systems: Case ... Ryad Amdoun, Lakhdar Khelifi, Majda Khelifi-Slaoui, Samia Am... Published: 18 April 2018
Iranian Journal of Biotechnology, doi: 10.21859/ijb.1339
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The use of the desirability function approach combined with the response surface methodology (RSM), also called Desirability Optimization Methodology (DOM), has been successfully applied to solve medical, chemical, and technological questions. It is particularly efficient for the determination of the optimal conditions in natural or industrial processes involving different factors leading to the antagonist responses. Surprisingly, DOM has never been applied to the research programs devoted to the study of plant responses to the complex environmental changes, and thus to biotechnological questions. In this article, DOM is used to study the response of Datura stramonium hairy roots (HRs), obtained by genetic transformation with Agrobacterium rhizogenes A4 strain, subjected to the jasmonate treatments. Antagonist effects on the growth and tropane alkaloid biosynthesis are confirmed. With a limited number of experimental conditions, it is shown that 0.06 mM jasmonic acid (JA) applied for 24 h leads to an optimal compromise. Hyoscyamine levels increase by up to 290% after 24 h and this treatment does not significantly inhibit biomass growth. It is thus demonstrated that the use of DOM can efficiently - with a minimized number of replicates - leads to the optimization of the biotechnological processes.
Article 0 Reads 1 Citation Investigation of Linum flavum (L.) Hairy Root Cultures for the Production of Anticancer Aryltetralin Lignans Sullivan Renouard, Cyrielle Corbin, Samantha Drouet, Barbara... Published: 26 March 2018
International Journal of Molecular Sciences, doi: 10.3390/ijms19040990
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Linum flavum hairy root lines were established from hypocotyl pieces using Agrobacterium rhizogenes strains LBA 9402 and ATCC 15834. Both strains were effective for transformation but induction of hairy root phenotype was more stable with strain ATCC 15834. Whereas similar accumulation patterns were observed in podophyllotoxin-related compounds (6-methoxy-podophyllotoxin, podophyllotoxin and deoxypodophyllotoxin), significant quantitative variations were noted between root lines. The influence of culture medium and various treatments (hormone, elicitation and precursor feeding) were evaluated. The highest accumulation was obtained in Gamborg B5 medium. Treatment with methyl jasmonate, and feeding using ferulic acid increased the accumulation of aryltetralin lignans. These results point to the use of hairy root culture lines of Linum flavum as potential sources for these valuable metabolites as an alternative, or as a complement to Podophyllum collected from wild stands.
Article 0 Reads 3 Citations Phenylpropanoid profiling reveals a class of hydroxycinnamoyl glucaric acid conjugates in Isatis tinctoria leaves Thi-Kieu-Oanh Nguyen, Arash Jamali, Eric Grand, Kris Morreel... Published: 01 December 2017
Phytochemistry, doi: 10.1016/j.phytochem.2017.09.007
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Article 0 Reads 2 Citations Isoquinoline alkaloid fractions of Fumaria officinalis : Characterization and evaluation of their antioxidant and antiba... Sabiha Khamtache-Abderrahim, Michelle Lequart-Pillon, Eric G... Published: 01 December 2016
Industrial Crops and Products, doi: 10.1016/j.indcrop.2016.09.016
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